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KMID : 0358220060330020115
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Korean Journal of Fertility and Sterility
2006 Volume.33 No. 2 p.115 ~ p.123
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Role of Integrin, FAK (Focal Adhesion Kinase) and ERK (Extracellular Signal Regulated Kinase) on the Suppressed Cell Proliferation of Endometrial Cancer Cells by GnRH (Gonadotropin-Releasing Hormone)
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Choi Jong-Rak
Park Dong-Uk
Choi Dong-Soon
Min Churl-K.
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Abstract
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Objective: To investigate new signal transduction cascade through integrin, FAK and ERK in the suppressed cell proliferation by GnRH-I and -II.
Method: Human endometrial cancer cells (HEC1A) were cultured under the following condition: DMEM/F12 (10% FBS). GnRH-I and -II were treated time (0, 5, 10, 15, 20, 30 min; 100 nM) and dose (10 nM or 100 nM; 20 min) dependent manner according to experimental purposes. Cell proliferation was measured using [3H] thymidine incorporation assay. Immunoblotting was utilized to detect proteins.
Results: GnRH-I and -II inhibited proliferation of HEC1A cells and induced expression of integrin β3. Phosphorylation of FAK and ERK were induced by GnRH-I and -II.
Conclusion: GnRH inhibited cell proliferation via the expression of integrin and FAK, ERK phosphorylation.
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KEYWORD
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GnRH, Integrin, FAK, ERK, Phosphorylation
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